| Player | # | Pos | HT | WT | Age | Exp | College |
|---|---|---|---|---|---|---|---|
| Brandon Allen | 17 | QB | 6-2 | 209 | 32 | 8 | Arkansas |
| Evan Anderson | 69 | DL | 6-3 | 326 | 22 | R | Florida Atlantic |
| Aaron Banks | 65 | OL | 6-5 | 325 | 27 | 4 | Notre Dame |
| Ben Bartch | 78 | OL | 6-6 | 315 | 26 | 5 | St. John’s (MN) |
| Robert Beal Jr. | 51 | DL | 6-4 | 250 | 25 | 2 | Georgia |
| Ronnie Bell | 10 | WR | 6-0 | 190 | 24 | 2 | Michigan |
| Tatum Bethune | 48 | LB | 6-0 | 299 | 23 | R | Florida State |
| Nick Bosa | 97 | DL | 6-4 | 266 | 27 | 6 | Ohio State |
| Jake Brendel | 64 | OL | 6-4 | 299 | 32 | 7 | UCLA |
| Ji’Ayir Brown | 27 | S | 5-11 | 202 | 24 | 2 | Penn State |
| Spencer Burford | 74 | OL | 6-4 | 300 | 24 | 3 | Texas-San Antonio |
| De’Vondre Campbell Sr. | 59 | LB | 6-4 | 232 | 31 | 9 | Minnesota |
| Maliek Collins | 99 | DL | 6-2 | 310 | 29 | 9 | Nebraska |
| Chris Conley | 18 | WR | 6-3 | 205 | 32 | 10 | Georgia |
| Jacob Cowing | 19 | WR | 5-9 | 171 | 23 | R | Arizona |
| Kalia Davis | 93 | DL | 6-2 | 310 | 26 | 3 | Central Florida |
| Khalil Davis | DL | 6-2 | 315 | 28 | 4 | Nebraska | |
| Joshua Dobbs | 5 | QB | 6-3 | 220 | 29 | 8 | Tennessee |
| Jordan Elliott | 92 | DL | 6-4 | 303 | 26 | 5 | Missouri |
| Demetrius Flannigan-Fowles | 45 | LB | 6-2 | 223 | 28 | 5 | Arizona |
| Leonard Floyd | 56 | DL | 6-3 | 240 | 32 | 9 | Georgia |
| Kevin Givens | 90 | DL | 6-1 | 285 | 27 | 5 | Penn State |
| Renardo Green | 0 | CB | 6-0 | 186 | 24 | R | Florida State |
| Yetur Gross-Matos | 94 | DL | 6-5 | 265 | 26 | 5 | Penn State |
| Isaac Guerendo | 31 | RB | 6-0 | 221 | 24 | R | Louisville |
| Jauan Jennings | 15 | WR | 6-3 | 212 | 27 | 4 | Tennessee |
| Kyle Juszczyk | 44 | FB | 6-2 | 235 | 33 | 12 | Harvard |
| George Kittle | 85 | TE | 6-4 | 250 | 31 | 8 | Iowa |
| Deommodore Lenoir | 2 | DB | 5-10 | 200 | 25 | 4 | Oregon |
| Jordan Mason | 24 | RB | 5-11 | 223 | 25 | 3 | Georgia Tech |
| Christian McCaffrey | 23 | RB | 5-11 | 210 | 28 | 8 | Stanford |
| Colton McKivitz | 68 | OL | 6-6 | 301 | 28 | 5 | West Virginia |
| Jake Moody | 4 | K | 6-1 | 210 | 24 | 2 | Michigan |
| Jaylon Moore | 76 | OL | 6-4 | 311 | 26 | 4 | Western Michigan |
| Malik Mustapha | 6 | S | 5-10 | 206 | 22 | R | Wake Forest |
| Pat O’Donnell | P | 6-4 | 220 | 33 | 10 | Miami | |
| George Odum | 30 | S | 6-1 | 202 | 31 | 7 | Central Arkansas |
| Sam Okuayinonu | 91 | DL | 6-1 | 269 | 26 | 2 | Maryland |
| Ricky Pearsall | 14 | WR | 6-3 | 192 | 24 | R | Florida |
| Taybor Pepper | 46 | LS | 6-4 | 245 | 30 | 7 | Michigan State |
| Dominick Puni | 77 | OL | 6-5 | 313 | 24 | R | Kansas |
| Brock Purdy | 13 | QB | 6-1 | 220 | 24 | 3 | Iowa State |
| Deebo Samuel Sr. | 1 | WR | 6-0 | 215 | 28 | 6 | South Carolina |
| Eric Saubert | 82 | TE | 6-5 | 248 | 30 | 7 | Drake |
| Jake Tonges | 88 | TE | 6-4 | 240 | 25 | 2 | California |
| Charvarius Ward | 7 | CB | 6-1 | 196 | 28 | 7 | Middle Tennessee State |
| Fred Warner | 54 | LB | 6-3 | 230 | 28 | 7 | Brigham Young |
| Trent Williams | 71 | T | 6-5 | 320 | 36 | 15 | Oklahoma |
| Brayden Willis | 9 | TE | 6-4 | 240 | 25 | 2 | Oklahoma |
| Dee Winters | 53 | LB | 5-11 | 227 | 24 | 2 | TCU |
| Rock Ya-Sin | 33 | CB | 5-11 | 195 | 28 | 6 | Temple |
| Isaac Yiadom | 22 | CB | 6-1 | 232 | 28 | 7 | Boston College |
| Nick Zakelj | 63 | OL | 6-6 | 316 | 25 | 3 | Fordham |
Reserve/Injured
| Player | # | Pos | HT | WT | Age | Exp | College |
|---|---|---|---|---|---|---|---|
| Brandon Aiyuk | 11 | WR | 6-0 | 200 | 26 | 5 | Arizona State |
| Jon Feliciano | 55 | OL | 6-4 | 325 | 32 | 10 | Miami |
| Javon Hargrave | 98 | DL | 6-2 | 305 | 31 | 9 | South Carolina State |
| Talanoa Hufanga | 29 | S | 6-0 | 200 | 24 | 4 | Southern California |
| Darrell Luter Jr. | 28 | CB | 6-0 | 190 | 24 | 2 | South Alabama |
| Elijah Mitchell | 25 | RB | 5-10 | 200 | 26 | 4 | Louisiana |
| Curtis Robinson | 36 | LB | 6-3 | 235 | 26 | 3 | Stanford |
| Ambry Thomas | 20 | CB | 6-0 | 190 | 25 | 4 | Michigan |
| Mitch Wishnowsky | 3 | P | 6-2 | 220 | 32 | 6 | Utah |
| Matthew Wright | 35 | K | 5-11 | 174 | 28 | 3 | UCF |
Reserve/Physically Unable to Perform
| Player | # | Pos | HT | WT | Age | Exp | College |
|---|---|---|---|---|---|---|---|
| Dre Greenlaw | 57 | LB | 6-0 | 230 | 27 | 6 | Arkansas |
| Drake Jackson | 95 | DL | 6-4 | 273 | 23 | 3 | Southern California |
Practice Squad
| Player | # | Pos | HT | WT | Age | Exp | College |
|---|---|---|---|---|---|---|---|
| Isaac Alarcon | 67 | OL | 6-7 | 320 | 26 | 1 | Tecnológico de Monterrey |
| Alex Barrett | 58 | DL | 6-2 | 250 | 30 | 2 | San Diego State |
| Russell Gage | WR | 6-0 | 184 | 28 | 7 | LSU | |
| Tashaun Gipson Sr. | S | 6-1 | 212 | 34 | 13 | Wyoming | |
| Jalen Graham | 50 | LB | 6-3 | 220 | 24 | 2 | Purdue |
| Chase Lucas | 26 | CB | 5-11 | 184 | 27 | 3 | Arizona State |
| Jaylen Mahoney | 38 | S | 5-11 | 200 | 24 | R | Vanderbilt |
| Nick McCloud | CB | 6-1 | 193 | 26 | 4 | Notre Dame | |
| Tanner Mordecai | 4 | QB | 6-2 | 218 | 25 | R | Wisconsin |
| Drake Nugent | 66 | OL | 6-1 | 298 | 23 | R | Michigan |
| Terique Owens | 83 | WR | 6-3 | 193 | 25 | R | Missouri State |
| Mason Pline | 89 | TE | 6-6 | 254 | 24 | R | Furman |
| Nesta Jade Silvera | 75 | DL | 6-2 | 315 | 24 | 2 | Arizona State |
| Patrick Taylor Jr. | 32 | RB | 6-2 | 217 | 26 | 4 | Memphis |
| Trent Taylor | 81 | WR | 5-8 | 178 | 30 | 7 | Louisiana Tech |
| DaShaun White | 47 | LB | 6-0 | 218 | 24 | 1 | Oklahoma |